Agilent
1200 series HPLC (High-Performance Liquid Chromatography) High-Performance Liquid Chromatography. High performance means high separation capability. The HPLC device is one of the most widely used devices for analytical separation purposes. Liquid carriers are used as mobile phases for the purpose of separation of substances in a mixture. The substances in the mixture are first dissolved in a solvent and then passed through a chromatographic column under high pressure. The separation of substances takes place in chromatographic columns. The conditions that make the widespread use of the system important are its sensitivity, selectivity, ability to perform quantitative determinations, and, most importantly, the ability to separate non-volatile and temperature-stable substances. We can show amino acids, proteins, nucleic acids, carbohydrates, medicinal substances, etc. as substances that can be analyzed by liquid chromatography. HPLC is widely used in food and beverage, environment, medicine, petrochemicals, agriculture, and many other fields. Basic Parts of HPLC Mobile Phase Reservoir Pump Injector (Autosampler) Column Detector (DAD, FLD, ELSD, UV, RID and MS) Software
Agilent HPLC 1200 Series: Precision and Performance in Liquid Chromatography
What is the Agilent HPLC 1200 Series?
The Agilent HPLC 1200 Series represents a line of High-Performance Liquid Chromatography (HPLC) systems renowned for their exceptional separation capabilities, sensitivity, and robust performance. These systems are optimized for a wide array of analytical applications.
How does an Agilent HPLC 1200 work?
Similar to general HPLC principles:
- A liquid mobile phase carries a dissolved sample mixture through a chromatographic column.
- The column contains a stationary phase that interacts differently with each component of the mixture.
- High pressure is used to force the mixture through the column, leading to separation.
- A detector identifies and measures the separated components.
Key Applications of Agilent HPLC 1200 Series
The Agilent 1200 Series excels in various industries:
- Food and Beverage: Quality control, ingredient analysis
- Environment: Pollutant detection, water quality monitoring
- Medicine: Drug development, purity testing, therapeutic monitoring
- Petrochemicals: Analyzing complex mixtures
- Agriculture: Pesticide residue testing
Essential Components of an Agilent HPLC 1200 System
- Mobile Phase Reservoir: Stores the liquid solvent
- Pump: Delivers the mobile phase at consistent pressure
- Injector (Autosampler): Introduces the sample into the mobile phase stream
- Column: The heart of the separation process
- Detector: Identifies and quantifies separated components (Common types: DAD, FLD, ELSD, UV, RID, MS)
- Software: Controls the system, collects and analyzes data
Why Choose the Agilent HPLC 1200 Series?
- High performance: Superior resolution, sensitivity, and speed
- Reliability: Robust, long-lasting instrumentation
- Modularity: Customizable configurations to suit specific needs